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Part:BBa_K2970002
Gate
This gate is a toehold switch system with which a gene of interest can be locked and regulated on a translational level using mRNA as regulator. After transcription, the mRNA of this gate forms a hairpin structure that hides the ribosome binding site and start codon of the gene of interest, thus translation can not be initiated (Figure 1B). A complementary part to the gate, a so called trigger, is needed to open the hairpin and release the ribosome binding site. In our case two triggers are needed that form a trigger complex to open the gate (BBa_K2970000 and BBa_K2970001). The affinity between the trigger complex and the gate is greater than that of the gate to itself (in the hairpin). A single trigger cannot open the gate because it has only half the required complementary sequence.
![](https://2019.igem.org/wiki/images/b/bc/T--Hamburg--PartsFigure11.jpeg)
![](https://2019.igem.org/wiki/images/4/49/T--Hamburg--Part_Figure6.jpg)
Results
For results visit page of BBa_K2980006.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
None |